INFLUENCE OF TRIBULUS TERRESTRIS EXTRACT ON BONE MORPHOLOGY AND BIOMECHANICS OF RAT FEMURS
DOI:
https://doi.org/10.61164/nkdrzg74Keywords:
Tribulus terrestris, phytotherapy, bone biomechanics, androgenic effects, testosterone, bone densityAbstract
Introduction: Tribulus terrestris (TT) is an herb credited as a food supplement and capable of providing metabolic gains, from the refinement of androgen levels and treatment of various diseases to the improvement of bone mineral content. Objective: to evaluate the influence of TT extract on the biomechanical and histomorphologic properties of rat femurs. Methodology: An experimental study was performed with Wistar rats, divided into two groups (n=10), Control (CT), treated with 0.5ml of 0.9% SF, orally, and Tribulus terrestris (TT), treated with TT extract in a dose of 50 mg/kg in a 0.5 ml solution, orally. After 60 days, the right femurs were dissected and the morphology and biomechanics were analyzed. Results: during the experiment, all animals gained weight, being the CT group gain (181±21) higher than the TT one (132±12). Morphometric dimension analyses did not reveal significant differences and interactions between CT and TT groups. The TT group presented significantly inconsistent data in the histomorphometric analysis of the trabecular and cortical area and thickness of the femurs, when compared to the CT group, showing larger dimensions upon consumption of the TT. The biomechanical analyses revealed remarkable differences, as the TT group needed a greater maximum force for rupture, displacement, extrinsic rigidity, elasticity, tension and deformation of the femurs until the fracture was evidenced. Conclusion: the consumption of TT had positive effects regarding the biomechanical properties, volume and thickness of the femurs, and in the trabecular area, suggesting a greater bone resistance to the fracture caused by the flexion test.
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ABDEL-ALL, S. R. et al. Phytochemical and Biological Evaluation of a Newly Designed Nutraceutical Self-Nanoemulsifying Self-Nanosuspension for Protection and Treatment of Cisplatin Induced Testicular Toxicity in Male Rats. Molecules, 26(2):408, jan. 2021. doi: 10.3390/molecules26020408. PMID: 33466804; PMCID: PMC7830605. DOI: https://doi.org/10.3390/molecules26020408
BERTOZZO, T. V. et al. Análise histológica do fêmur de camundongos suíços fêmeas ovariectomizadas e não ovariectomizadas suplementadas com extrato de Tribulus terrestris L. SALUSVITA, Bauru, v.38, n.2, p.369-379, 2019.
DALMOLIN, F. et al. Biomecânica óssea e ensaios biomecânicos: fundamentos teóricos. Cienc. Rural, Santa Maria, v. 43, n. 9, p. 1675-1682, Sept. 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000900022&lng=en&nrm=iso>. Acessado em: 05 Mai 2021. https://doi.org/10.1590/S0103-84782013000900022. DOI: https://doi.org/10.1590/S0103-84782013000900022
DINIZ, J. S. et al. Propriedades mecânicas do tecido ósseo: uma revisão bibliográfica. In: IX Encontro Latino Americano de Iniciação Científica e V Encontro Latino Americano de Pós-Graduação: 2005, Universidade do Vale do Paraíba.
FIDELIS, M. et al. Perspectiva de tratamento da osteoporose dos maxilares por meio de fitoterápicos: uma revisão da literatura. Rev Thêma et Scientia, v. 10, n.2, jul./dez. 2020.
GAUTHAMAN, K.; GANESAN, A. P. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction--an evaluation using primates, rabbit and rat. Phytomedicine, 15(1-2):44-54, jan. 2008. doi: 10.1016/j.phymed.2007.11.011. PMID: 18068966. DOI: https://doi.org/10.1016/j.phymed.2007.11.011
GOLDS, G.; HOUDEK, D.; ARNASON, T. Male Hypogonadism and Osteoporosis: The Effects, Clinical Consequences, and Treatment of Testosterone Deficiency in Bone Health. Int J Endocrinol, 2017:4602129, 2017. doi:10.1155/2017/4602129 DOI: https://doi.org/10.1155/2017/4602129
HUANG, T. H. et al. Effects of different exercise modes on mineralization, structure, and biomechanical properties of growing bone. J Appl Physiol (1985), 95(1):300-7, jul. 2003. doi: 10.1152/japplphysiol.01076.2002. Epub 2003 Feb 28. PMID: 12611764. DOI: https://doi.org/10.1152/japplphysiol.01076.2002
KANG, S. Y. et al. Effects of the Fruit Extract of Tribulus terrestris on Skin Inflammation in Mice with Oxazolone-Induced Atopic Dermatitis through Regulation of Calcium Channels, Orai-1 and TRPV3, and Mast Cell Activation. Evid Based Complement Alternat Med. 2017, 2017:8312946. doi: 10.1155/2017/8312946. Epub 2017 Nov 14. PMID: 29348776; PMCID: PMC5733837. DOI: https://doi.org/10.1155/2017/8312946
KIM, H.J. et al. Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells. J Ethnopharmacol, 136(1):197-203, jun. 2011. doi: 10.1016/j.jep.2011.04.060. Epub 2011 Apr 28. PMID: 21549825. DOI: https://doi.org/10.1016/j.jep.2011.04.060
LAMMERS, A. R.; GERMAN, R. Z.; LIGHTFOOT, P. S. The impact of muscular dystrophy on limb bone growth and scaling in mice. Acta Anat (Basel), 162(4):199-208, 1998. doi: 10.1159/000046435. PMID: 9831769. DOI: https://doi.org/10.1159/000046435
LOK, S. Does the use of Testosterone Enanthate as a Form of Doping in Sports Cause Early Closure of Epiphyseal in Bones? Int. J. Morphol., Temuco, v.33, n.4, p.1201-1204, dez. 2015. Disponible en <https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400001&lng=es&nrm=iso>. Accedido en 01 feb. 2021. http://dx.doi.org/10.4067/S0717-95022015000400001. DOI: https://doi.org/10.4067/S0717-95022015000400001
MA, Y.; GUO, Z.; MANG, X. Tribulus terrestris extracts alleviate muscle damage and promote anaerobic performance of trained male boxers and its mechanisms: Roles of androgen, IGF-1, and IGF binding protein-3. J Sport Health Sci, 6(4): 474-481, dec. 2017. doi: 10.1016/j.jshs.2015.12.003. Epub 2015 Dec 14. PMID: 30356644; PMCID: PMC6189260. DOI: https://doi.org/10.1016/j.jshs.2015.12.003
MARQUES, M. A. A. et al. Osteoprotective Effects of Tribulus terrestris L.: Relationship Between Dehydroepiandrosterone Levels and Ca2+-Sparing Effect. J Med Food, 22(3):241-247, mar. 2019. doi: 10.1089/jmf.2018.0090. PMID: 30888914. DOI: https://doi.org/10.1089/jmf.2018.0090
NAKAGAKI, W. R. et al. Mechanical, biochemical and morphometric alterations in the femur of mdx mice. Bone, 48(2):372-9, 2011. doi: 10.1016/j.bone.2010.09.011. Epub 2010 Sep 17. PMID: 20850579. DOI: https://doi.org/10.1016/j.bone.2010.09.011
NEYCHEY, V.; MITEV, V. Pro-sexual and androgen enhancing effects of Tribulus terrestris L.: Fact or Fiction. J Ethnopharmacol, 179:345-55, feb. 2016. doi: 10.1016/j.jep.2015.12.055. Epub 2015 Dec 28. PMID: 26727646. DOI: https://doi.org/10.1016/j.jep.2015.12.055
PARHAM, S. et al. Antioxidant, Antimicrobial and Antiviral Properties of Herbal Materials. Antioxidants (Basel), 9(12):1309, dec. 2020. doi: 10.3390/antiox9121309. PMID: 33371338; PMCID: PMC7767362. DOI: https://doi.org/10.3390/antiox9121309
PARK, Y. J. et al. Effects of Tribulus terrestris on monosodium iodoacetateinduced osteoarthritis pain in rats. Mol Med Rep, 16(4):5303-5311, oct. 2017. doi: 10.3892/mmr.2017.7296. Epub 2017 Aug 21. PMID: 28849084; PMCID: PMC5647062. DOI: https://doi.org/10.3892/mmr.2017.7296
POKRYWKA, A. et al. Insights into Supplements with Tribulus Terrestris used by Athletes. J Hum Kinet. 41:99-105, jul. 2014. doi: 10.2478/hukin-2014-0037. PMID: 25114736; PMCID: PMC4120469. DOI: https://doi.org/10.2478/hukin-2014-0037
QURESHI, A.; NAUGHTON, D. P.; PETROCZI, A. A systematic review on the herbal extract Tribulus terrestris and the roots of its putative aphrodisiac and performance enhancing effect. J Diet Suppl, 11(1):64-79, mar. 2014. doi: 10.3109/19390211.2014.887602. PMID: 24559105. DOI: https://doi.org/10.3109/19390211.2014.887602
SALGADO, R. M. et al. Effect of oral administration of Tribulus terrestris extract on semen quality and body fat index of infertile men. Andrologia, 49(5), jun. 2017. doi: 10.1111/and.12655. Epub 2016 Jul 12. PMID: 27401787. DOI: https://doi.org/10.1111/and.12655
SANTOS, H.O.; HOWELL, S.; TEIXEIRA, F.J. Beyond tribulus (Tribulus terrestris L.): The effects of phytotherapics on testosterone, sperm and prostate parameters. J Ethnopharmacol., 235:392-405, 2019. doi:10.1016/j.jep.2019.02.033 DOI: https://doi.org/10.1016/j.jep.2019.02.033
SOARES, E. V. et al. Effects of alcohol and nicotine on the mechanical resistance of bone and bone neoformation around hydroxyapatite implants. J Bone Miner Metab, 28(1):101-7, 2010. doi: 10.1007/s00774-009-0115-1. Epub 2009 Aug 8. PMID: 19669082. DOI: https://doi.org/10.1007/s00774-009-0115-1
STEFÃNESCU, R. et al. A Comprehensive Review of the Phytochemical, Pharmacological, and Toxicological Properties of Tribulus terrestris L. Biomolecules, 10(5):752, may 2020. doi: 10.3390/biom10050752. PMID: 32408715; PMCID: PMC7277861. DOI: https://doi.org/10.3390/biom10050752
VALE, F. B. C. et al. Efficacy of Tribulus Terrestris for the treatment of premenopausal women with hypoactive sexual desire disorder: a randomized double-blinded, placebo-controlled trial. Gynecol Endocrinol, 34(5):442-445, may 2018. doi: 10.1080/09513590.2017.1409711. Epub 2017 Nov 27. PMID: 29172782. DOI: https://doi.org/10.1080/09513590.2017.1409711
VAN DER LOOS, M. et al. Development of hip bone geometry during gender-affirming hormone therapy in transgender adolescents resembles that of the experienced gender when pubertal suspension is started in early puberty. J Bone Miner Res, jan. 2021. doi: 10.1002/jbmr.4262. Epub ahead of print. PMID: 33507568. DOI: https://doi.org/10.1002/jbmr.4262
ZHU, W. et al. A review of traditional pharmacological uses, phytochemistry, and pharmacological activities of Tribulus terrestris. Chem Cent J, 11(1):60, jul. 2017. doi: 10.1186/s13065-017-0289-x. PMID: 29086839; PMCID: PMC5503856. DOI: https://doi.org/10.1186/s13065-017-0289-x
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Copyright (c) 2025 JOABE OZIEL CARDOSO CRUZ, GABRIEL LESSA DE SOUZA MAIA, ISABELE, THIAGO DONIZETH DA SILVA, MARGARIDA PEREIRA SANTOS, ROMULO DIAS NOVAES, FLAVIA DA RÉ GUERRA, GERALDO JOSÉ MEDEIROS FERNANDES, MÁRCIA CRISTINA BIZINOTTO DE ASSUNÇÃO, JOSE ANTONIO DIAS GARCIA, EVELISE ALINE
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