LIPID MODULATION IN MEAT EMULSIONS USING SIMPLE AND GELLED OIL-IN-WATER EMULSION
DOI:
https://doi.org/10.66104/6q6jst82Keywords:
Meat emulsion, Mechanically deboned meat, Texture, Lipid profile, Lipid oxidationAbstract
Emulsified meat products formulated with a high content of mechanically deboned chicken meat (MDCM) are widely consumed in Brazil. Therefore, improving their nutritional profile and understanding how structured oils affect their physicochemical properties is of practical relevance. This study compared the incorporation of simple and gelled oil-in-water emulsions based on an olive and chia oil blend as pork fat replacers in meat emulsion model systems with high MDCM content. Sodium caseinate (SC) and soy protein isolate (SPI) were used as emulsifying agents, while carrageenan served as the gelling agent. The gelled emulsion stabilized with SC resulted in the highest emulsion stability, whereas the treatment containing the non-emulsified oil blend exhibited the lowest stability. No significant differences were observed between the remaining treatments and the control formulated with pork back fat. Meat emulsions containing simple and gelled emulsions stabilized with SC also showed improved oxidative stability compared with the other oil-containing treatments. Compressive resistance increased with the incorporation of both simple and gelled emulsions, whereas pH and protein and lipid contents did not differ among treatments. Overall, both simple and gelled emulsions enhanced emulsion stability and rheological properties in meat systems with high MDCM content. However, oil-in-water emulsions stabilized with SC provided superior oxidative stability.
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ABDEL-RAZEK, A. G.; et al. Omega fatty acid-balanced oil formula and enhancing its oxidative stability by encapsulation with whey protein concentrate. Food Bioscience, [S. l.], v. 50, p. 101975, 2022. DOI: https://doi.org/10.1016/j.fbio.2022.101975
AOAC. Official methods of analysis of AOAC International. 18th. ed. Gaithersburg, USA: AOAC International, 2005.
AOCS. Official methods and recommended practices of the American Oil Chemists’ Society. Champaign: AOCS Press, 2009.
ASUMING-BEDIAKO, N. et al. Effects of replacing pork backfat with emulsified vegetable oil on fatty acid composition and quality of UK-style sausages. Meat Science, [S. l.], v. 96, n. 1, p. 187–194, 2014. DOI: 10.1016/j.meatsci.2013.06.031. DOI: https://doi.org/10.1016/j.meatsci.2013.06.031
BAYARRI, S.; et al. Influence of texture on the temporal perception of sweetness of gelled systems. Food Research International, [S. l.], v. 40, p. 900–908, 2007. DOI: 10.1016/j.foodres.2007.03.003. DOI: https://doi.org/10.1016/j.foodres.2007.03.003
BLOUKAS, I.; HONIKEL, K. O. The Influence of Mincing and Temperature of Storage on the Oxidation of Pork Back Fat and Its Effect on Water- and Fat-Binding in Finely Comminuted Batters. Meat Science, [S. l.], v. 32, p. 215–227, 1992. DOI: https://doi.org/10.1016/0309-1740(92)90108-G
BOLGER, Z.; BRUNTON, N. P.; MONAHAN, F. J. Impact of inclusion of flaxseed oil (pre-emulsified or encapsulated) on the physical characteristics of chicken sausages. Journal of Food Engineering, [S. l.], v. 230, p. 39–48, 2018. DOI:
10.1016/j.jfoodeng.2018.02.026. DOI: https://doi.org/10.1016/j.jfoodeng.2018.02.026
BRAZIL. Instrução Normativa N° 4, de 21 de março de 2000. Diário Oficial da União, n° 66, seção 1, 2000, Brasília.
DELGADO-PANDO, G. et al. Low-fat frankfurters formulated with a healthier lipid combination as functional ingredient: Microstructure, lipid oxidation, nitrite content, microbiological changes and biogenic amine formation. Meat Science, [S. l.], v. 89, n. 1, p. 65–71, 2011. DOI: 10.1016/j.meatsci.2011.03.022. DOI: https://doi.org/10.1016/j.meatsci.2011.03.022
DOS SANTOS, M. et al. Pork skin-based emulsion gels as animal fat replacers in hot-dog style sausages. LWT, [S. l.], v. 132, p. 109845, 2020. DOI: 10.1016/j.lwt.2020.109845. DOI: https://doi.org/10.1016/j.lwt.2020.109845
ELIAS, R. J.; KELLERBY, S. S.; DECKER, E. A. Antioxidant activity of proteins and peptides. Critical Reviews in Food Science and Nutrition, [S. l.], v. 48, n. 5, p. 430–441, 2008. DOI: 10.1080/10408390701425615. DOI: https://doi.org/10.1080/10408390701425615
EU. Commission Regulation (EU) No 432/2012. Official Journal of the European Union, , 2012.
FARAJI, H. D.; MCCLEMENTS, J.; DECKER, E. A. Role of Continuous Phase Protein on the Oxidative Stability of Fish Oil-in-Water Emulsions. J. Agric. Food Chem., [S. l.], v. 52, p. 4558–4564, 2004. DOI: https://doi.org/10.1021/jf035346i
FERREIRA, D. F. Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, [S. l.], v. 38, n. 2, p. 109–112, 2014. DOI: 10.1590/s1413-70542014000200001. DOI: https://doi.org/10.1590/S1413-70542014000200001
FORELL, S. C. P. et al. Effect of type of emulsifiers and antioxidants on oxidative stability, colour and fatty acid profile of low-fat beef burgers enriched with unsaturated fatty acids and phytosterols. MESC, [S. l.], v. 86, n. 2, p. 364–370, 2010. DOI: 10.1016/j.meatsci.2010.05.015. DOI: https://doi.org/10.1016/j.meatsci.2010.05.015
FREIRE, M. et al. Double emulsions to improve frankfurter lipid content : impact of perilla oil and pork backfat. [S. l.], n. January, 2016. DOI: 10.1002/jsfa.7163. DOI: https://doi.org/10.1002/jsfa.7163
GORZYNIK-DEBICKA, M. et al. Potential Health Benefits of Olive Oil and Plant Polyphenols. International Journal of Molecular Sciences, [S. l.], v. 19, n. 3, p. 686, 2018. DOI: 10.3390/ijms19030686. DOI: https://doi.org/10.3390/ijms19030686
HARTMAN, L.; LAGO, R. C. A. Rapid preparation of fatty acid methyl esters from lipids. Lab.Pract., [S. l.], v. 22, n. 7, p. 475–476, 1973.
HORITA, C. N. et al. Textural, microstructural and sensory properties of reduced sodium frankfurter sausages containing mechanically deboned poultry meat and blends of chloride salts. Food Research International, [S. l.], v. 66, p. 29–35, 2014. DOI: 10.1016/j.foodres.2014.09.002. DOI: https://doi.org/10.1016/j.foodres.2014.09.002
HU, M. D.; MCCLEMENTS, J.; DECKER, E. A. Lipid Oxidation in Corn Oil-in-Water Emulsions Stabilized by Casein, Whey Protein Isolate, and Soy Protein Isolate. J. Agric. Food Chem., [S. l.], v. 51, p. 1696–1700, 2003. DOI: https://doi.org/10.1021/jf020952j
HUGHES, E.; COFRADES, S.; TROY, D. J. Effects of fat level, oat fibre and carrageenan on frankfurters formulated with 5, 12 and 30% fat. Meat Science, [S. l.], v. 45, n. 3, p. 273–281, 1997. DOI: 10.1016/S0309-1740(96)00109-X. DOI: https://doi.org/10.1016/S0309-1740(96)00109-X
JIMÉNEZ-COLMENERO, F. et al. Influence of emulsified olive oil stabilizing system used for pork backfat replacement in frankfurters. Food Research International, [S. l.], v. 43, p. 2068–2076, 2010. DOI: 10.1016/j.foodres.2010.06.010. DOI: https://doi.org/10.1016/j.foodres.2010.06.010
KIM, T-K et al. Effects of replacing pork fat with grape seed oil and gelatine/alginate for meat emulsions. Meat Science, [S. l.], v. 163, p. 108079, 2020. DOI: 10.1016/j.meatsci.2020.108079. DOI: https://doi.org/10.1016/j.meatsci.2020.108079
KRIS-ETHERTON, P. M.; FLEMING, J. A. Emerging Nutrition Science on Fatty Acids and Cardiovascular Disease: Nutritionists’ Perspectives. Advances in Nutrition: An International Review Journal, [S. l.], v. 6, n. 3, p. 326S-337S, 2015. DOI: 10.3945/an.114.006981. DOI: https://doi.org/10.3945/an.114.006981
KRIS-ETHERTON, P. M.; PETERSEN, K.; VAN HORN, L. Convincing evidence supports reducing saturated fat to decrease cardiovascular disease risk. BMJ Nutrition, Prevention & Health, [S. l.], v. 1, n. 1, p. 23–26, 2018. DOI: 10.1136/bmjnph-2018-000009. DOI: https://doi.org/10.1136/bmjnph-2018-000009
LI-HUA, P.; BAJINKA, O. Processed Meat Health Risks: Pathways and Dietary Solutions. The Journal of Nutrition, [S. l.], v. 155, n. 11, p. 3584–3594, 2025. DOI: 10.1016/j.tjnut.2025.08.030. DOI: https://doi.org/10.1016/j.tjnut.2025.08.030
LIMA, T. L. S. et al. Vegetable oils in emulsified meat products: a new strategy to replace animal fat. Food Science and Technology, [S. l.], v. 42, 2022. DOI: 10.1590/fst.103621. DOI: https://doi.org/10.1590/fst.103621
MARINELI, S. et al. Chemical characterization and antioxidant potential of Chilean chia seeds and oil ( Salvia hispanica L .). LWT - Food Science and Technology, [S. l.], v. 59, n. 2, p. 1304–1310, 2014. DOI: 10.1016/j.lwt.2014.04.014. DOI: https://doi.org/10.1016/j.lwt.2014.04.014
NETTLETON, J. A. et al. Saturated Fat Consumption and Risk of Coronary Heart Disease and Ischemic Stroke: A Science Update. Annals of Nutrition and Metabolism, [S. l.], v. 70, n. 1, p. 26–33, 2017. DOI: 10.1159/000455681. DOI: https://doi.org/10.1159/000455681
PAGLARINI, C. S. et al. Functional emulsion gels as pork back fat replacers in Bologna sausage. Food Structure, [S. l.], v. 20, n. March 2018, p. 100105, 2019. DOI: 10.1016/j.foostr.2019.100105. DOI: https://doi.org/10.1016/j.foostr.2019.100105
PAGLARINI, C. S; MARTINI, S.; POLLONIO, M. A. R. Using emulsion gels made with sonicated soy protein isolate dispersions to replace fat in frankfurters. LWT, [S. l.], v. 99, n. October 2018, p. 453–459, 2019. DOI: 10.1016/j.lwt.2018.10.005. DOI: https://doi.org/10.1016/j.lwt.2018.10.005
PARASKEVOPOULOU, D.; BOSKOU, D.; PARASKEVOPOULOU, A. Oxidative stability of olive oil-lemon juice salad dressings stabilized with polysaccharides. Food Chemistry, [S. l.], v. 101, n. 3, p. 1197–1204, 2007. DOI: 10.1016/j.foodchem.2006.03.022. DOI: https://doi.org/10.1016/j.foodchem.2006.03.022
PEREIRA, A. G. T. et al. Effects of the addition of mechanically deboned poultry meat and collagen fibers on quality characteristics of frankfurter-type sausages. Meat Science, [S. l.], v. 89, n. 4, p. 519–525, 2011. DOI: 10.1016/j.meatsci.2011.05.022. DOI: https://doi.org/10.1016/j.meatsci.2011.05.022
PEREIRA, P. A. P. et al. Rheological behavior of functional sugar-free guava preserves: Effect of the addition of salts. Food Hydrocolloids, [S. l.], v. 31, n. 2, p. 404–412, 2013. DOI: 10.1016/j.foodhyd.2012.11.014. DOI: https://doi.org/10.1016/j.foodhyd.2012.11.014
PINTADO, T. et al. Chia and oat emulsion gels as new animal fat replacers and healthy bioactive sources in fresh sausage formulation. Meat Science, [S. l.], v. 135, n. July 2017, p. 6–13, 2018. DOI: 10.1016/j.meatsci.2017.08.004. DOI: https://doi.org/10.1016/j.meatsci.2017.08.004
POYATO, C. et al. Optimization of a gelled emulsion intended to supply ω -3 fatty acids into meat products by means of response surface methodology. Meat Science, [S. l.], v. 98, n. 4, p. 615–621, 2014. DOI: 10.1016/j.meatsci.2014.06.016. DOI: https://doi.org/10.1016/j.meatsci.2014.06.016
RAHARJO, S.; SOFOS, J. N.; SCHMIDT, G. R. Improved Speed, Specificity, and Limit of Determination of an Aqueous Acid Extraction Thiobarbituric Acid-C18 Method for Measuring Lipid Peroxidation in Beef. J. Agrlc. Food Chm, [S. l.], v. 40, p. 2182–2185, 1992. DOI: https://doi.org/10.1021/jf00023a027
SALCEDO-SANDOVAL, L. et al. Filled hydrogel particles as a delivery system for n-3 long chain PUFA in low-fat frankfurters: Consequences for product characteristics with special reference to lipid oxidation. Meat Science, [S. l.], v. 110, p. 160–168, 2015. DOI: 10.1016/j.meatsci.2015.07.013. DOI: https://doi.org/10.1016/j.meatsci.2015.07.013
SERDAROĞLU, M.; NACAK, B.; KARABIYIKOĞLU, M. Effects of Beef Fat Replacement with Gelled Emulsion Prepared with Olive Oil on Quality Parameters of Chicken Patties. Korean Journal for Food Science of Animal Resources, [S. l.], v. 37, n. 3, p. 376–384, 2017. DOI: 10.5851/kosfa.2017.37.3.376. DOI: https://doi.org/10.5851/kosfa.2017.37.3.376
SERDAROĞLU, M.; ÖZTÜRK, B.; URGU, M. Emulsion characteristics, chemical and textural properties of meat systems produced with double emulsions as beef fat replacers. Meat Science, [S. l.], v. 117, p. 187–195, 2016. DOI: 10.1016/j.meatsci.2016.03.012. DOI: https://doi.org/10.1016/j.meatsci.2016.03.012
TANG, M-X. et al. Rheological and structural properties of sodium caseinate as influenced by locust bean gum and κ-carrageenan. Food Hydrocolloids, [S. l.], v. 112, p. 106251, 2021. DOI: 10.1016/j.foodhyd.2020.106251. DOI: https://doi.org/10.1016/j.foodhyd.2020.106251
TRINDADE, M. A.; JOSEFINA, C.; CASTILLO, C. Mortadella sausage formulations with mechanically separated layer hen meat preblended with antioxidants. Sci. Agric. (Piracicaba, Braz.), [S. l.], v. 63, n. 3, p. 240–245, 2006. DOI: https://doi.org/10.1590/S0103-90162006000300005
VIEIRA, S. A.; ZHANG, G.; DECKER, E. A. Biological Implications of Lipid Oxidation Products. Journal of the American Oil Chemists’ Society, [S. l.], v. 94, n. 3, p. 339–351, 2017. DOI: 10.1007/s11746-017-2958-2. DOI: https://doi.org/10.1007/s11746-017-2958-2
WARAHO, T.; MCCLEMENTS, D. J.; DECKER, E. A. Mechanisms of lipid oxidation in food dispersions. Trends in Food Science & Technology, [S. l.], v. 22, n. 1, p. 3–13, 2011. DOI: 10.1016/j.tifs.2010.11.003. DOI: https://doi.org/10.1016/j.tifs.2010.11.003
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