Use of spent mushroom substrate (SMS) in new production cycles and evaluation of the antioxidant activity of Lentinus crinitus and Pleurotus spp.
DOI:
https://doi.org/10.66104/m58yet98Keywords:
Antioxidant; basidiomycete; microbial succession; waste valorization.Abstract
Lentinus crinitus and Pleurotus spp. are mushrooms that can be produced in lignocellulosic agro-industrial by-products. After mushroom production, the substrate containing the mycelium constitutes the spent mushroom substrate (SMS). Its reuse in new production cycles generates prospects for a more efficient production chain. The SMS from L. crinitus was used to produce Pleurotus spp. and and the reverse path was taken, to verify the possibility of SMS reuse. The antioxidant activity of the SMS generated in the two cycles of cultivation and the mushrooms harvested was evaluated. Cultivation in SMS decreased productivity, biological efficiency, and substrate consumption. P. djamor and P. ostreatus produced in SMS from L. crinitus showed a reduction in productivity of 4.4 and 4.2 times, respectively. Biological efficiency followed the same decreasing trend. The microbial succession reduced the SMS antioxidant activity by the FRAP (ferric reducing antioxidant power) and BCLA (beta-carotene/linoleic acid co-oxidation system) methods. Only the antioxidant activity by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method of SMS increased after the second cultivation cycle. Mushrooms produced in secondary cultivation have higher DPPH antioxidant activity than in primary cultivation. L. crinitus produced in succession to P. djamor presented a 2.5-fold reduction in inhibitory concentration (IC50) compared to mushrooms from the primary cultivation. The growth in SMS also increased mushroom FRAP activity. L. crinitus grown in SMS of P. djamor, and SMS of P. ostreatus showed the highest antioxidant activities by FRAP, seven times and 6.3 times superior to reference control. Only the antioxidant activity by BCLA of P. ostreatus mushrooms grown in SMS of L. crinitus was significantly higher than the mushrooms from primary cultivation. Despite the reduction in productivity, the use of SMS in a new substrate composition increased the antioxidant activity of mushrooms. The SMS produce basidiocarps with higher content of compounds with antioxidant activity.
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Copyright (c) 2026 Olavo Bilac Quaresma de Oliveira Filho , Miria Benetati Delgado Bertéli, Nelson Barros Colauto, Giani Andrea Linde Colauto, Perseu da Silva Aparicio, William Kalhy Silva Xavier, Carlos Henrique Medeiros de Abreu, Juliana Silveira do Valle
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