DETERMINATION OF PHOSPHORUS CONCENTRATION IN OXISOL YELLOW BY DIGITAL IMAGES IN THE MUNICIPALITY OF ITACOATIARA-AM
DOI:
https://doi.org/10.61164/rmnm.v6i1.2497Keywords:
Imagens digitais, Scanner, Fósforo no soloAbstract
The objective of this work was to quantify the concentration of phosphorus available in the yellow latosol - type soil of the pineapple crop by means of a colorimetric method based on image analysis. The images of soil extracts were obtained using commercial scanners and free software. The determination of phosphorus is based on the reaction between orthophosphate ions (PO 3 ) and mo 2 lybdate (MoO4 ) in strongly acid medium, forming the molybdophosphoric complex, which is reduced by ascorbic acid to molybdenum blue. After color formation, absorbance is measured using a UV/VIS spectrophotometer at 660 nm. Simultaneously the extracts / phosphorous patterns were transferred to a microplate on the scanner, scanned and analyzed with the ImageJ program. The vector norm is used as the analytical response, which is related to the concentration of phosphorus (mg/dm³) that is determined from the linear model of the standard curve. Samples were collected in the Village of the Remanso New, municipality of Itacoatiara-AM, during the winter and summer periods of the region. Extraction of available phosphorus in the extracts was performed with Melich 1 extractor in the proportion of 1:10. Filtration of extracts, volume study of standard solutions on the microplate and area of the analyzed images contributed to the final analysis results. A relationship between the time of analysis of the patterns and the correlation of the standard curves. The concentrations of phosphorus in the samples collected in the dry period were higher than those collected in the rainy season, both by the reference method and by the proposed method. LD and LQ values were lower by digital images. After application of the T-test it was found that there was no significant difference between the two methodologies for a 95% confidence level (calculated = 0.4705 and t critical = 2.7764) and the relative standard deviations were less than 3.0 %.
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ALCARDE, J. C.; GUIDOLIN, J. A.; LOPES, A. S. Os adubos e a eficiência das adubações. 3ª ed. (Boletim Técnico 3). São Paulo. ANDA. 1998.
BENEDETTI, L. P. DOS S. et al. A digital image analysis method for quantification of sulfite in beverages. Anal. Methods, v. 7, n. 18, p. 7568- 7573, 2015. DOI: https://doi.org/10.1039/C5AY01372K
COCHRANE, T.T.; SANCHEZ, P.A. Land resources, soils properties and their management in the Amazon region: A state of knowledge report. In: HECHT, S.B., ed. Amazon land use research. Cali, CIAT, 1982. 70p.
DOMINGUEZ, M. A.; CENTURIÓN, M. E. Application of digital images to determine color in honey samples from Argentina. Microchemical Journal, v. 118, p. 110–114, 2015. DOI: https://doi.org/10.1016/j.microc.2014.08.002
FILHO, JULIANO PASSARETTI; PETRUCI, JOÃO FLÁVIO DA SILVEIRA; CARDOSO, ARNALDO ALVES. Development of a simple method for determination of NO2 in airusing digital scanner images. Talanta, v. 140, p. 73–80, 2015. DOI: https://doi.org/10.1016/j.talanta.2015.03.009
GAIÃO, E. N.; MARTINS, V. L.; LYRA, W. S.; ALMEIDA, L. F.; SILVA, E. C.; ARAÚJO, M. C. U. Digital image-based titrations. Anal. Chim. Acta 570 (2006), 283-290. DOI: https://doi.org/10.1016/j.aca.2006.04.048
LEPSCH, Igo. F. Formação e conservação dos solos. 2ª ed. São Paulo: Oficina de Textos, 2010. 216p.
LIMA, R. A. C. et al. Digital movie-based on automatic titrations. Talanta, v. 147, p. 226–232, 2016. DOI: https://doi.org/10.1016/j.talanta.2015.09.053
LYRA, W. S. et al. Digital image-based flame emission spectrometry. Talanta, v. 77; p. 1584, 2009. DOI: https://doi.org/10.1016/j.talanta.2008.09.057
MASAWAT, P.; HARFIELD, A.; NAMWONG, A. An iPhone-based digital image colorimeter for detecting tetracycline in milk. FOOD CHEMISTRY, v. 184, p. 23–29, 2015. DOI: https://doi.org/10.1016/j.foodchem.2015.03.089
MAIA, M.A.; MARMOS, J.L(ORG). Geodiversidade do estado do Amazonas. Manaus. CPRM, 2010. 275 p.
MALAVOLTA, Eurípedes. Manual de nutrição mineral de plantas. São Paulo: CERES, 2006. 638p.
MALÉZIEUX, E.BARTHOLOMEW, D.P. Plant Nutrition. In: Bartholomew, D.P.; Paul, R.E., Rohrbach, K.G. The Pineapple - Botany, Production and Uses. Honolulu: CABI Publishing. 2003. cap. 7, p.143-165. DOI: https://doi.org/10.1079/9780851995038.0143
NETO, G. F. S.; FONSECA, A.; BRAGA, J.W. B. Classificação De Águas Minerais Baseada Em Imagens Digitais Obtidas Por Smartphones. Química Nova, v. 39, n. 7, p. 876–881, 2016.
RIBEIRO, F.A.L.; FERREIRA, M.M.C.; MORANO, S.C.; SILVA, L.R. SCHNEIDER, R.P. Planilha de validação: Uma nova ferramenta para estimar figuras de mérito na validação de métodos analíticos univariados. Química Nova, 1:164-171, 2008. DOI: https://doi.org/10.1590/S0100-40422008000100029
SANTOS, LÊNIO DA SILVA; SILVA, LEANDRO SOUZA; GRIEBELER, GUSTAVO. Ácido ascórbico como agente redutor para determinação de fósforo por colorimetria. Ciência Rural, Santa Maria, Rio Grande do Sul, Brasil, v.44, n.6, p.1015-1018, jun, 2014. DOI: https://doi.org/10.1590/S0103-84782014000600010
SILVA, FÁBIO CESAR. Manual de análise químicas de solos, plantas e fertilizantes. 2. Ed. rev. ampl. EMBRAPA. Brasília, DF. Embrapa Informação Tecnológica, 2009.
SOUZA, FABRÍCIO RIBEIRO de, et al. Avaliação de dispositivos de captura de imagens digitais para detecção colorimétrica em microzonas impressas. Quim. Nova v. 37, n. 7, p. 1171-1176, 2014
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