AVALIAÇÃO DOS HORMONAS SEXUAIS FEMENINAS EN LOS TEJIDOS PERIODONTALES DE RATAS OBESAS SOMETIDAS A PERIODONTITE EXPERIMENTAL
DOI:
https://doi.org/10.66104/1epwtw35Palabras clave:
Obesidad; Enfermedad periodontal; Tejido óseo.Resumen
Objetivo: El objetivo de este estudio fue evaluar el efecto de la influencia hormonal sobre los tejidos periodontales de ratas adultas en la fase de proestro del ciclo estral, con obesidad inducida experimentalmente a través de una dieta de cafetería, asociada o no a enfermedad periodontal. Diseño: Para este estudio, 20 ratas hembras adultas en ciclo en la fase de proestro del ciclo estral se dividieron en un grupo control (CON), un grupo de ligadura (LIG), un grupo de cafetería (CAF) y un grupo de cafetería y ligadura (CAFLIG). A los 75 días de edad, los animales de los grupos LIG y CAFLIG recibieron una ligadura alrededor del primer molar inferior derecho, que actuó como irritante gingival durante 30 días, favoreciendo la acumulación de placa bacteriana y el consecuente desarrollo de enfermedad periodontal. Después de la eutanasia, a los 105 días de edad, se recolectó sangre del tronco encefálico derecho para determinar las concentraciones de estradiol y progesterona utilizando kits específicos; y la disección de la hemimandíbula derecha, que se sometió a análisis histológico. Los datos obtenidos se analizaron y evaluaron mediante pruebas ANOVA y de Tukey. Resultados: Los resultados demuestran una disminución en la concentración de hormonas sexuales femeninas cuando se asociaron la obesidad y la enfermedad periodontal; de igual manera, se observó una marcada resorción ósea y cambios en la morfología del tejido óseo en el grupo CAF/LIG. Conclusión: Con base en los resultados obtenidos, este estudio puede concluir que la disminución de las hormonas sexuales femeninas asociada con la obesidad y la enfermedad periodontal interfirió negativamente en el tejido óseo mandibular.
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AGEEL, R.; ABAALKHAIL, B.; NATTO, Z. S. Effect of hormone replacement therapy on periodontal health in post-menopausal women in Jeddah, Saudi Arabia. BMC Women's Health, v. 25, n. 1, p. 383, 2025. DOI: https://doi.org/10.1186/s12905-025-03757-5
ARIAS, C. et al. Enhancing adipose tissue functionality in obesity: senotherapeutics, autophagy and cellular senescence as a target. Biological Research, v. 57, n. 1, ago. 2024. DOI: https://doi.org/10.1186/s40659-024-00531-z
BIAMONTE, E. et al. Bone Health in Metabolic Syndrome-Is It a Neglected Aspect of Dysmetabolic-Related Diseases? Journal of Clinical Medicine, v. 14, n. 16, p. 5785, ago. 2025. DOI: https://doi.org/10.3390/jcm14165785
BORELLI, A. Envelhecimento ósseo: osteoporose. In: CARVALHO FILHO, E. T.; PAPALÉO NETTO, M. Geriatria: fundamentos, clínica e terapêutica. São Paulo: Atheneu, cap. 22, p. 297-308, 1994.
BOYAPATI, R. et al. Influence of female sex hormones in different stages of women on periodontium. Journal of Mid-life Health, v. 12, n. 4, p. 263, 2021. DOI: https://doi.org/10.4103/jmh.jmh_142_21
BRAY, G. A.; YORK, D. A. Hypothalamic and genetic obesity in experimental animals: na autonomic and endocrine hypothesis. Physiology Reviews, v. 59, n. 3, p. 719-809, 1979. DOI: https://doi.org/10.1152/physrev.1979.59.3.719
CHEN, R.; ARMAMENTO-VILLAREAL, R. Obesity and Skeletal Fragility. The Journal of Clinical Endocrinology & Metabolism, jul. 2023. DOI: https://doi.org/10.1210/clinem/dgad415
COATMELLEC-TAGLIONI, G. et al. Gender difference in diet-induced obesity hypertension: implication of renal alpha2-adrenergic receptors. American Journal of Hypertension, v. 15, p. 143-149, 2002. DOI: https://doi.org/10.1016/S0895-7061(01)02269-5
CUTOLO, M. et al. Estrogens, the immune response and autoimmunity. Clinical and Experimental Rheumatology, v. 13, n. 2, p. 217-226, 2007.
DIAS, P. et al. Relationship between obesity and periodontal disease: a cross-sectional study. Journal of Periodontology, v. 84, n. 3, 2023.
DUAN, F. et al. Deciphering Endocrine Function of Adipose Tissue and Its Significant Influences in Obesity-Related Diseases Caused by Its Dysfunction. Differentiation, v. 141, p. 100832, dez. 2024. DOI: https://doi.org/10.1016/j.diff.2024.100832
DUARTE, P. M. et al. Effect of an estrogen-deficient state and its therapy on bone loss resulting from an experimental periodontitis in rats. Journal of Periodontal Research, v. 39, p. 107-110, 2004. DOI: https://doi.org/10.1111/j.1600-0765.2004.00714.x
HAMDY, O. Obesity: Background, Pathophysiology, Etiology. Medscape, 2025. Disponível em: http://emedicine.medscape.com/article/123702-overview. Acesso em: 3 mar. 2026.
HERBISON, A. E. Estrogen positive feedback to gonadotropin-releasing hormone (GnRH) neurons in the rodent: the case for the rostral periventricular area of the third ventricle (RP3V). Brain Research Reviews, v. 57, n. 2, p. 277-287, 2008. DOI: https://doi.org/10.1016/j.brainresrev.2007.05.006
HSU, S.-H.; CHEN, L.-R.; CHEN, K.-H. Primary Osteoporosis Induced by Androgen and Estrogen Deficiency: The Molecular and Cellular Perspective on Pathophysiological Mechanisms and Treatments. International Journal of Molecular Sciences, v. 25, n. 22, p. 12139, 2024. DOI: https://doi.org/10.3390/ijms252212139
KAGIOS, C. et al. Cafeteria diet and caloric restriction affect metabolic but not behavioral characteristics in male Wistar rats. Physiology & Behavior, v. 288, p. 114731, jan. 2025. DOI: https://doi.org/10.1016/j.physbeh.2024.114731
KAUFFMAN, A. S. Neuroendocrine mechanisms underlying estrogen positive feedback and the LH surge. Frontiers in Neuroscience, v. 16, p. 953252, 2022. DOI: https://doi.org/10.3389/fnins.2022.953252
KLEINERT, M. et al. Animal models of obesity and diabetes mellitus. Nature Reviews Endocrinology, v. 14, n. 3, p. 140–162, mar. 2018. DOI: https://doi.org/10.1038/nrendo.2017.161
KURYŁOWICZ, A. Estrogens in Adipose Tissue Physiology and Obesity-Related Dysfunction. Biomedicines, v. 11, n. 3, p. 690, fev. 2023. DOI: https://doi.org/10.3390/biomedicines11030690
LEE, A. A.; DEN, L. J. Metabolic impact of endogenously produced estrogens by adipose tissue in females and males across the lifespan. Frontiers in Endocrinology, v. 16, out. 2025. DOI: https://doi.org/10.3389/fendo.2025.1682231
LIU, Y. et al. The secretory function of adipose tissues in metabolic regulation. Life Metabolism, jan. 2024. DOI: https://doi.org/10.1093/lifemeta/loae003
LLADO, I. et al. Protein and amino acid intake in cafeteria fed obese rats. Physiology & Behavior, v. 58, p. 513-519, 1995. DOI: https://doi.org/10.1016/0031-9384(95)00081-S
MESEGUER, A.; PUCHE, C.; CABERO, A. Sex steroid biosynthesis in white adipose tissue. Hormone Metabolic Research, v. 34, p. 731-736, 2002. DOI: https://doi.org/10.1055/s-2002-38249
MIGLIACCIO, S. et al. Glucocorticoid-induced osteoporosis: an osteoblastic disease. Aging Clinical Experimental Research, v. 19, p. 5-10, 2007.
MOURA-GREC, P. G. et al. Obesity and periodontitis: systematic review and meta-analysis. CAERN, 2023.
PALANISAMY, S. The impact of estrogen on periodontal tissue integrity and inflammation: a mini review. Frontiers in Dental Medicine, v. 6, p. 1455755, 2025. DOI: https://doi.org/10.3389/fdmed.2025.1455755
PRADA, P. O. et al. Western diet modulates insulin signaling, c-Jun n-Terminal kinase activity, and insulin receptor substrate-1ser307 phosphorylation in a tissue-specific fashion. Endocrinology, v. 146, p. 1576-1587, 2005. DOI: https://doi.org/10.1210/en.2004-0767
REID, I. R. Relationship between fat and bone. Osteoporosis International, v. 9, p. 595-606, 2008. DOI: https://doi.org/10.1007/s00198-007-0492-z
RITTER, C. et al. Soluble epoxide hydrolase inhibition preserves alveolar bone in experimental periodontitis with estrogen deficiency. Frontiers in Immunology, v. 16, p. 1708504, 2025. DOI: https://doi.org/10.3389/fimmu.2025.1708504
ROSSETTI, B. R. et al. Effects of estrogen deficiency on the progression of apical periodontitis: a systematic review of preclinical studies. Archives of Oral Biology, v. 142, p. 105496, 2022. DOI: https://doi.org/10.1016/j.archoralbio.2022.105496
SCHICHT, M. et al. Articular cartilage chondrocytes express aromatase and use enzymes involved in estrogen metabolism. Arthritis Research & Therapy, v. 16, n. 2, p. R93, 2014. DOI: https://doi.org/10.1186/ar4539
SCLAFANI, A.; SPRINGER, D. Dietary in obesity in adult rats: similarities to hypothalamic and human syndromes. Physiology & Behavior, v. 17, p. 461-471, 1976. DOI: https://doi.org/10.1016/0031-9384(76)90109-8
SEGAL, M. M.; BELL, J.; ABRAMS, G. M. Hypothalamic or central obesity associated with an early rise in plasma insulin concentration. Archives of Neurology, v. 48, p. 429-431, 1991. DOI: https://doi.org/10.1001/archneur.1991.00530160099022
SMITH, M. S.; FREEMAN, M. E.; NEILL, J. D. The control of progesterone secretion during the estrous cycle and early pseudopregnancy in the rat. Endocrinology, v. 96, p. 219-226, 1975. DOI: https://doi.org/10.1210/endo-96-1-219
SON, Y. et al. Immunometabolic crosstalk in adipose tissue remodeling: mechanisms and therapeutic perspectives. Journal of Obesity & Metabolic Syndrome, v. 34, n. 4, p. 344–361, out. 2025. DOI: https://doi.org/10.7570/jomes25080
SØRENSEN, M. G. et al. Estrogen directly attenuates human osteoclastogenesis, but has no effect on resorption by mature osteoclasts. DNA Cell Biology, v. 25, n. 8, p. 475-483, ago. 2006. DOI: https://doi.org/10.1089/dna.2006.25.475
SPEAKMAN, J. R. Use of high-fat diets to study rodent obesity as a model of human obesity. International Journal of Obesity, v. 43, n. 8, p. 1491–1492, abr. 2019. DOI: https://doi.org/10.1038/s41366-019-0363-7
SUZUKI, N. et al. Hypothalamic obesity due to hydrocephalus caused by aqueductal stenosis. Journal of Neurology Neurosurgery and Psychiatry, v. 53, p. 1102-1103, 1990. DOI: https://doi.org/10.1136/jnnp.53.12.1102
SZEJNFELD, V. L. Alterações ósseas: fisiopatologia, diagnóstico e tratamento. In: FERNANDES, C. E. Menopausa: diagnóstico e tratamento. 1. ed. São Paulo: Seguimento, 2003.
TSUCHIDA, J. et al. Effects of estrogen and mechanical loading on cultured cells derived from mandibular condylar cartilage. Scientific Reports, v. 15, n. 1, p. 23470, 2025. DOI: https://doi.org/10.1038/s41598-025-07770-4
VENKEN, K. et al. Sex hormones, their receptors and bone health. Osteoporosis International, v. 19, p. 1517–1525, 2008. DOI: https://doi.org/10.1007/s00198-008-0609-z
WEI, S. et al. Obesity and diabetes in transgenic mice expressing pro SAAS. Journal of Endocrinology, v. 180, p. 357-368, 2004. DOI: https://doi.org/10.1677/joe.0.1800357
WORLD HEALTH ORGANIZATION (WHO). Obesity and overweight, May 2012. Seção Media Centre. Disponível em: http://www.who.int/mediacentre/factsheets/fs311/en/index.html. Acesso em: 29 jun. 2013.
WORLD HEALTH ORGANIZATION (WHO). Obesity and Overweight. 2025/2026. Disponível em: https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight. Acesso em: fev. 2026.
ZHANG, Y.-Y. et al. Insights and implications of sexual dimorphism in osteoporosis. Bone Research, v. 12, n. 1, p. 1–30, fev. 2024. DOI: https://doi.org/10.1038/s41413-023-00306-4
ZHAO, L. J. et al. Correlation of obesity and osteoporosis: effect of fat mass on the determination of osteoporosis. Journal of Bone and Mineral Research, v. 23, p. 17-29, 2008. DOI: https://doi.org/10.1359/jbmr.070813
ZHAO, L. J. et al. Obesity, Bone Loss, and Periodontitis: The Interlink. Biomolecules,. Journal of Bone and Mineral Research, v.12, n.7, p.865, 2022. DOI: https://doi.org/10.3390/biom12070865
ZUCKER, I. The mixed legacy of the rat estrous cycle. Biology of Sex Differences, v. 14, n. 1, set. 2023. DOI: https://doi.org/10.1186/s13293-023-00542-7
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Derechos de autor 2026 Poliana de Fátima Biederman, Alana Zenilda Thomaz Sacht, Vitoria Bortolon Jassniker, Mateus Pacer de Lima, Carlos Augusto Nassar, Rose Meire Costa, Sara Cristina Sagae, Patricia Oehlmeyer Nassar
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