ANALYSIS OF GRAM-NEGATIVE BACTERIA IN COMMON-USE BATHROOMS OF A HIGHER EDUCATION INSTITUTION IN TERESINA-PI
DOI:
https://doi.org/10.61164/ec702t74Keywords:
Gram-negative bacteria, Biosafety, Bacterial contamination, HygieneAbstract
Public restrooms in higher education institutions are environments prone to microbiological contamination due to the intense flow of people, high humidity, and frequent contact with high-touch surfaces such as faucets and sinks. The presence of Gram-negative bacteria in these areas poses a significant public health risk, given their intrinsic resistance to various antimicrobials and their potential to cause opportunistic infections. Therefore, this study aimed to evaluate the presence and prevalence of Gram-negative bacteria in restrooms at a higher education institution in Teresina, Piauí. This experimental study involved collecting samples from surfaces in different restrooms within the institution. After bacterial isolation on specific culture media, the colonies were subjected to MALDI-TOF mass spectrometry analysis for microbiological identification. The results were organized according to frequency of occurrence and characterization of the isolated species. All samples showed bacterial growth, confirming the widespread contamination of external surfaces. Acinetobacter baumannii predominated, isolated in 26 samples, followed by Enterobacter sp. and Pseudomonas aeruginosa, both detected in eight samples. The persistence of these species reflects their high intrinsic resistance to antimicrobials, as well as flaws in hygiene and ventilation protocols, which favor colonization in humid environments. The identified bacterial diversity, combined with the multidrug-resistant profile, suggests the need for more rigorous biosafety measures. Therefore, shared restrooms represent significant reservoirs of Gram-negative bacteria with potential health risks. These results reinforce the importance of intensifying cleaning protocols, implementing continuous microbiological monitoring, and promoting health education, ensuring a reduction in indirect transmission through surfaces and greater protection for the academic community.
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ABNEY, S. E. et al. Transmission of Viruses from Restroom Use: A Quantitative Microbial Risk Assessment. Food and environmental virology, v. 16, n. 1, p. 65–78, 19 fev. 2024. DOI: https://doi.org/10.1007/s12560-023-09580-1
ARAÚJO, B. C. de et al. Prevenção e controle de resistência aos antimicrobianos na Atenção Primária à Saúde: evidências para políticas. Ciência & Saúde Coletiva, v. 27, p. 299-314, jan. 2022. DOI: https://doi.org/10.1590/1413-81232022271.22202020
BARBOSA, K. H. et al. Impacto do MALDI-TOF no diagnóstico da Sepse: uma revisão integrativa. Brazilian Journal of Development, v. 7, n. 6, p. 58556–58574, 2021. DOI: https://doi.org/10.34117/bjdv7n6-313
BUSH, Larry M. Considerações gerais sobre bactérias Gram-negativas. Manual MSD Versão Saúde para a Família. Revisado em jun. 2024. Disponível em: https://www.msdmanuals.com/pt-br/casa/infecções/infecções-bacterianas-bactérias-gram-negativas/considerações-gerais-sobre-bactérias-gram-negativas. Acesso em: 11 set. 2024.
FILHA, H. M. D. C. O. et al. Ocorrência de agentes infecciosos em torneiras dos banheiros de uma instituição de ensino superior. Arquivos Médicos dos Hospitais e da Faculdade de Ciências Médicas da Santa Casa de São Paulo, v. 63, n. 1, p. 25, 8 Maio 2018. DOI: https://doi.org/10.26432/1809-3019.2018.63.1.25
GERBA, C. P. et al. Bacterial Contamination of Public and Household Restrooms, and Implications for the Potential Risk of Norovirus Transmission. Hygiene, v. 5, n. 3, p. 27–27, 2 jul. 2025. DOI: https://doi.org/10.3390/hygiene5030027
GOODMAN, A. L.; COOK, G. J.; GOH, V. Imaging in the investigation and management of Staphylococcus aureus bacteraemia: a role for advanced imaging techniques. Journal of Hospital Infection, v. 105, n. 2, p. 234–241, jun. 2020. DOI: https://doi.org/10.1016/j.jhin.2020.01.007
HERVÉ, R. C. et al. Impact of different hand-drying methods on surrounding environment: aerosolization of virus and bacteria, and transfer to surfaces. Journal of Hospital Infection, v. 147, p. 197–205, 1 maio 2024. DOI: https://doi.org/10.1016/j.jhin.2024.03.005
IBRAHIM, K. et al. Surveillance of Bacterial Load and Multidrug-Resistant Bacteria on Surfaces of Public Restrooms. International Journal of Environmental Research and Public Health, v. 21, n. 5, p. 574, 30 abr. 2024. DOI: https://doi.org/10.3390/ijerph21050574
LEE, M. C. J.; THAM, K. W. Public toilets with insufficient ventilation present high cross infection risk. Scientific Reports, v. 11, n. 1, 18 out. 2021. DOI: https://doi.org/10.1038/s41598-021-00166-0
LOPES, H. V.; TAVARES, W. Diagnóstico das infecções do trato urinário. Revista da Associação Médica Brasileira, v. 51, p. 306-308, dez. 2005. DOI: https://doi.org/10.1590/S0104-42302005000600008
MADIGAN, M. T.; BENDER, K. S.; BUCKLEY, D. H.; SATTLEY, W. M.; STAHL, D. A. Biology of microorganisms. 15. ed. Pearson, 2018.
MARTINS, C. C. et al. Earthquake, floods and changing land use history: A 200-year overview of environmental changes in Selenga River basin as indicated by n-alkanes and related proxies in sediments from shallow lakes. Science of The Total Environment, v. 873, p. 162245–162245, 1 maio 2023. DOI: https://doi.org/10.1016/j.scitotenv.2023.162245
MUSHABATI N.A.; SAMUTELA M.T.; YAMBA K.; et al. Bacterial contamination of 448 mobile phones of healthcare workers at the University Teaching Hospital, Lusaka, 449 Zambia. Infection prevention in practice, v. 3, n. 2, p. 100126–100126, 2021. DOI: https://doi.org/10.1016/j.infpip.2021.100126
NEMATIAN, J. et al. A survey of public restrooms microbial contamination in Tehran city, capital of Iran, during 2019. Journal of Family Medicine and Primary Care, v. 9, n. 6, p. 3131, 2020. DOI: https://doi.org/10.4103/jfmpc.jfmpc_300_20
NG, M. K.; MONT, M. A.; BONUTTI, P. M. Clinical and Environmental Harms of Quaternary Ammonium Disinfectants and the Promise of Ultraviolet-C (UV-C) Alternatives: A Narrative Review. Cureus, 13 maio 2025. DOI: https://doi.org/10.7759/cureus.84022
PEREIRA, M. S. et al. A infecção hospitalar e suas implicações para o cuidar da enfermagem. Texto Contexto Enferm., Florianópolis, v. 14, n. 2, p. 250-257, abr./jun. 2005. DOI: https://doi.org/10.1590/S0104-07072005000200013
PRICE, L. et al. Systematic review on factors influencing the effectiveness of alcohol-based hand rubbing in healthcare. Antimicrobial Resistance & Infection Control, v. 11, n. 1, 24 jan. 2022. DOI: https://doi.org/10.1186/s13756-021-01049-9
REYNOLDS, K. A. et al. Comparison of electric hand dryers and paper towels for hand hygiene: a critical review of the literature. Journal of Applied Microbiology, v. 130, n. 1, 14 ago. 2020. DOI: https://doi.org/10.1111/jam.14796
SHAMSIZADEH, Z. et al. Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections. Environmental Health and Preventive Medicine, v. 22, n. 1, 8 maio 2017. DOI: https://doi.org/10.1186/s12199-017-0653-4
SILVA, R. A. et al. Resistência a Antimicrobianos: a formulação da resposta no âmbito da saúde global. Saúde Debate, Rio de Janeiro, v. 44, n. 126, p. 607-623, jul.-set. 2020. DOI: https://doi.org/10.1590/0103-1104202012602
SOUZA, L. L. B.; FERREIRA, L. C. Contaminação microbiológica em Smartphones. Revista Vértices, v. 20, n. 2, p. 207–212, 30 ago. 2018. DOI: https://doi.org/10.19180/1809-2667.v20n22018p207-212
TORTORA, G.J.; FUNKE, B.R.; CASE, C.L. Microbiologia. Artmed, São Paulo, 12 ed. 2017.
VAZQUEZ-PERTEJO, Maria T. Introdução ao diagnóstico laboratorial das doenças infecciosas. Revisado/corrigido: out. 2022. Disponível em: https://www.msdmanuals.com/pt-br/profissional/doenças-infecciosas/diagnóstico-laboratorial-das-doenças-infecciosas/introdução-ao-diagnóstico-laboratorial-das-doenças-infecciosas. Acesso em: 11 set. 2024.
VIEIRA, D. A. P.; FERNANDES, N. C. A. Q. Microbiologia Geral. Inhumas: Instituto Federal de Educação, Ciência e Tecnologia de Goiás (IFG), 2012. Capítulo 8 - Provas bioquímicas e cultura de microrganismos. Disponível em: https://www.ufsm.br/app/uploads/sites/413/2018/12/05_microbiologia_geral.pdf. Acesso em: 30 out. 2024.
ZIEMAH, J.; ULLRICH, M. S.; KUHNERT, N. Development of Hot Trub and Coffee Silverskin Phytoextracts for Sustainable Aerosol Disinfectant Application. Foods, v. 14, n. 14, p. 2496–2496, 16 jul. 2025. DOI: https://doi.org/10.3390/foods14142496
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