ASPECTS RELATED TO THE MICROALGAE HARVESTING PROCESS
DOI:
https://doi.org/10.66104/tfmmf458Palavras-chave:
coagulação; eletrocoagulação; filtração; flotação; colheita; microalgas.Resumo
Microalgae are present in the aquatic environment and produce bioproducts of great added value (e.g. carbohydrates, lipids, fatty acids, pigments, protein) through the conversion of solar energy into chemical energy by fixing atmospheric CO2. Therefore, microalgae biomass has many potential applications in several industries, for example, cosmetics, pharmaceuticals, agricultural biopesticides, biofuel production, and food supplements. However, microalgae harvesting involves energy consumption and high operating costs (about 30 % of the total production cost). Thus, this paper aims to review the main achievements of microalgae harvesting processes, their efficiencies, and the main advantages and disadvantages of each harvesting method. Coagulant dosage, mixing time, and velocity gradient are parameters that affect the efficiency of microalgae harvesting. Therefore, the optimal conditions of these variables will be presented in this review article. On the other hand, coagulation, followed by flotation, is influenced by attraction-repulsion forces between cells and the coagulant agent. Factors such as cell size, viability, density, possible cell damage, strain properties, sedimentation rate, the salt concentration of each algae species of microalgae, energy demand, final product, and the reuse of culture medium determine if will be necessary one or methods combined for harvesting the microalgae biomass. However, coagulation and flocculation are still relevant for the harvesting of algae cells.
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Referências
Ajala, S.O., Alexander, M.L., 2020. Application of bio-based alkali to induce flocculation of microalgae biomass. Biomass Bioenergy 132, 105431. https://doi.org/10.1016/J.BIOMBIOE.2019.105431 DOI: https://doi.org/10.1016/j.biombioe.2019.105431
Álvarez, X., Jiménez, A., Cancela, Á., Valero, E., Sánchez, Á., 2021. Harvesting freshwater algae with tannins from the bark of forest species: Comparison of methods and pelletization of the biomass obtained. Chemosphere 268, 129313. https://doi.org/10.1016/J.CHEMOSPHERE.2020.129313 DOI: https://doi.org/10.1016/j.chemosphere.2020.129313
Bhatt, A., Khanchandani, M., Rana, M.S., Prajapati, S.K., 2022. Techno-economic analysis of microalgae cultivation for commercial sustainability: A state-of-the-art review. J Clean Prod 370, 133456. https://doi.org/10.1016/J.JCLEPRO.2022.133456 DOI: https://doi.org/10.1016/j.jclepro.2022.133456
Chen, C.Y., Yeh, K.L., Aisyah, R., Lee, D.J., Chang, J.S., 2011. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review. Bioresour Technol 102, 71–81. https://doi.org/10.1016/J.BIORTECH.2010.06.159 DOI: https://doi.org/10.1016/j.biortech.2010.06.159
Cheng, Y.L., Juang, Y.C., Liao, G.Y., Tsai, P.W., Ho, S.H., Yeh, K.L., Chen, C.Y., Chang, J.S., Liu, J.C., Chen, W.M., Lee, D.J., 2011. Harvesting of Scenedesmus obliquus FSP-3 using dispersed ozone flotation. Bioresour Technol 102, 82–87. https://doi.org/10.1016/J.BIORTECH.2010.04.083 DOI: https://doi.org/10.1016/j.biortech.2010.04.083
Daneshvar, E., Zarrinmehr, M.J., Kousha, M., Bhatnagar, A., 2020. Performance evaluation of different harvesting methods and cultivation media on the harvesting efficiency of microalga and their fatty acids profile. Fuel 280, 118592. https://doi.org/10.1016/J.FUEL.2020.118592 DOI: https://doi.org/10.1016/j.fuel.2020.118592
Demir-Yilmaz, I., Ftouhi, M.S., Balayssac, S., Guiraud, P., Coudret, C., Formosa-Dague, C., 2023. Bubble functionalization in flotation process improve microalgae harvesting. Chemical Engineering Journal 452, 139349. https://doi.org/10.1016/J.CEJ.2022.139349 DOI: https://doi.org/10.1016/j.cej.2022.139349
Dias, A., Borges, A.C., Rosa, A.P., Martins, M.A., 2021. Green coagulants recovering Scenedesmus obliquus: An optimization study. Chemosphere 262, 127881. https://doi.org/10.1016/J.CHEMOSPHERE.2020.127881 DOI: https://doi.org/10.1016/j.chemosphere.2020.127881
El Gamal, A.A., 2010. Biological importance of marine algae. Saudi Pharm J 18, 1–25. https://doi.org/10.1016/J.JSPS.2009.12.001 DOI: https://doi.org/10.1016/j.jsps.2009.12.001
El-taweel, R.M., Mohamed, N., Alrefaey, K.A., Husien, S., Abdel-Aziz, A.B., Salim, A.I., Mostafa, N.G., Said, L.A., Fahim, I.S., Radwan, A.G., 2023. A review of coagulation explaining its definition, mechanism, coagulant types, and optimization models; RSM, and ANN. Current Research in Green and Sustainable Chemistry 6, 100358. https://doi.org/10.1016/J.CRGSC.2023.100358 DOI: https://doi.org/10.1016/j.crgsc.2023.100358
Esteves, A.F., Almeida, C.J., Ana Luísa, G., Pires, J.C., 2020. Microalgae harvesting techniques. Handbook of Microalgae-Based Processes and Products: Fundamentals and Advances in Energy, Food, Feed, Fertilizer, and Bioactive Compounds 225–281. https://doi.org/10.1016/B978-0-12-818536-0.00010-5 DOI: https://doi.org/10.1016/B978-0-12-818536-0.00010-5
Fuad, N., Omar, R., Kamarudin, S., Harun, R., Idris, A., Wan, W.A., 2018. Mass harvesting of marine microalgae using different techniques. Food and Bioproducts Processing 112, 169–184. https://doi.org/10.1016/J.FBP.2018.10.006 DOI: https://doi.org/10.1016/j.fbp.2018.10.006
Gerulová, K., Kucmanová, A., Sanny, Z., Garaiová, Z., Seiler, E., Čaplovičová, M., Čaplovič, L., Palcut, M., 2022. Fe3O4-PEI Nanocomposites for Magnetic Harvesting of Chlorella vulgaris, Chlorella ellipsoidea, Microcystis aeruginosa, and Auxenochlorella protothecoides. Nanomaterials 2022, Vol. 12, Page 1786 12, 1786. https://doi.org/10.3390/NANO12111786 DOI: https://doi.org/10.3390/nano12111786
Ghazvini, M., Kavosi, M., Sharma, R., Kim, M., 2022. A review on mechanical-based microalgae harvesting methods for biofuel production. Biomass Bioenergy 158, 106348. https://doi.org/10.1016/J.BIOMBIOE.2022.106348 DOI: https://doi.org/10.1016/j.biombioe.2022.106348
Gorin, K. V., Sergeeva, Y.E., Butylin, V. V., Komova, A. V., Pojidaev, V.M., Badranova, G.U., Shapovalova, A.A., Konova, I.A., Gotovtsev, P.M., 2015. Methods coagulation/flocculation and flocculation with ballast agent for effective harvesting of microalgae. Bioresour Technol 193, 178–184. https://doi.org/10.1016/J.BIORTECH.2015.06.097 DOI: https://doi.org/10.1016/j.biortech.2015.06.097
Gouveia, L., 2011. Microalgae as a Feedstock for Biofuels. Microalgae as a Feedstock for Biofuels 1–69. https://doi.org/10.1007/978-3-642-17997-6_1 DOI: https://doi.org/10.1007/978-3-642-17997-6_1
Khatib, W.A., Ayari, A., Yasir, A.T., Talhami, M., Das, P., Quadir, M.A., Hawari, A.H., 2021. Enhancing the electrocoagulation process for harvesting marine microalgae (Tetraselmis sp.) using interdigitated electrodes. J Environ Manage 292, 112761. https://doi.org/10.1016/J.JENVMAN.2021.112761 DOI: https://doi.org/10.1016/j.jenvman.2021.112761
Khor, W.H., Kang, H.S., Lim, J.W., Iwamoto, K., Tang, C.H.H., Goh, P.S., Quen, L.K., Shaharuddin, N.M.R. Bin, Lai, N.Y.G., 2022. Microalgae cultivation in offshore floating photobioreactor: State-of-the-art, opportunities and challenges. Aquac Eng 98, 102269. https://doi.org/10.1016/J.AQUAENG.2022.102269 DOI: https://doi.org/10.1016/j.aquaeng.2022.102269
Kurniawan, S.B., Ahmad, A., Imron, M.F., Abdullah, S.R.S., Othman, A.R., Hasan, H.A., 2022a. Potential of microalgae cultivation using nutrient-rich wastewater and harvesting performance by biocoagulants/bioflocculants: Mechanism, multi-conversion of biomass into valuable products, and future challenges. J Clean Prod 365, 132806. https://doi.org/10.1016/J.JCLEPRO.2022.132806 DOI: https://doi.org/10.1016/j.jclepro.2022.132806
Kurniawan, S.B., Imron, M.F., Chik, C.E.N.C.E., Owodunni, A.A., Ahmad, A., Alnawajha, M.M., Rahim, N.F.M., Said, N.S.M., Abdullah, S.R.S., Kasan, N.A., Ismail, S., Othman, A.R., Hasan, H.A., 2022b. What compound inside biocoagulants/bioflocculants is contributing the most to the coagulation and flocculation processes? Science of The Total Environment 806, 150902. https://doi.org/10.1016/J.SCITOTENV.2021.150902 DOI: https://doi.org/10.1016/j.scitotenv.2021.150902
Laamanen, C.A., Ross, G.M., Scott, J.A., 2016. Flotation harvesting of microalgae. Renewable and Sustainable Energy Reviews 58, 75–86. https://doi.org/10.1016/J.RSER.2015.12.293 DOI: https://doi.org/10.1016/j.rser.2015.12.293
Labeeuw, L., Commault, A.S., Kuzhiumparambil, U., Emmerton, B., Nguyen, L.N., Nghiem, L.D., Ralph, P.J., 2021. A comprehensive analysis of an effective flocculation method for high quality microalgal biomass harvesting. Science of The Total Environment 752, 141708. https://doi.org/10.1016/J.SCITOTENV.2020.141708 DOI: https://doi.org/10.1016/j.scitotenv.2020.141708
Leite, L. de S., Hoffmann, M.T., Daniel, L.A., 2019. Coagulation and dissolved air flotation as a harvesting method for microalgae cultivated in wastewater. Journal of Water Process Engineering 32, 100947. https://doi.org/10.1016/J.JWPE.2019.100947 DOI: https://doi.org/10.1016/j.jwpe.2019.100947
Li, S., Hu, T., Xu, Y., Wang, J., Chu, R., Yin, Z., Mo, F., Zhu, L., 2020. A review on flocculation as an efficient method to harvest energy microalgae: Mechanisms, performances, influencing factors and perspectives. Renewable and Sustainable Energy Reviews 131, 110005. https://doi.org/10.1016/J.RSER.2020.110005 DOI: https://doi.org/10.1016/j.rser.2020.110005
Lucakova, S., Branyikova, I., Kovacikova, S., Pivokonsky, M., Filipenska, M., Branyik, T., Ruzicka, M.C., 2021. Electrocoagulation reduces harvesting costs for microalgae. Bioresour Technol 323, 124606. https://doi.org/10.1016/J.BIORTECH.2020.124606 DOI: https://doi.org/10.1016/j.biortech.2020.124606
Luo, J., Fang, Z., Smith, R.L., 2014. Ultrasound-enhanced conversion of biomass to biofuels. Prog Energy Combust Sci 41, 56–93. https://doi.org/10.1016/J.PECS.2013.11.001 DOI: https://doi.org/10.1016/j.pecs.2013.11.001
Lv, J., Liu, G., Feng, J., Liu, Q., Nan, F., Liu, X., Xie, S., 2020. Harvesting biomass of an oil-rich microalga Parachlorella kessleri TY02 by ferric chloride: Effects on harvesting efficiency, lipid production and municipal wastewater treatment. J Environ Manage 273, 111128. https://doi.org/10.1016/J.JENVMAN.2020.111128 DOI: https://doi.org/10.1016/j.jenvman.2020.111128
Maneechote, W., Cheirsilp, B., Angelidaki, I., Suyotha, W., Boonsawang, P., 2023. Chitosan-coated oleaginous microalgae-fungal pellets for improved bioremediation of non-sterile secondary effluent and application in carbon dioxide sequestration in bubble column photobioreactors. Bioresour Technol 372, 128675. https://doi.org/10.1016/J.BIORTECH.2023.128675 DOI: https://doi.org/10.1016/j.biortech.2023.128675
Marinho, Y.F., Oliveira, C.Y.B., Malafaia, C.B., Cahú, T.B., Oliveira, A.P.S., Napoleão, T.H., Bezerra, R.S., Paiva, P.G., Gálvez, A.O., 2022. A circular approach for the efficient recovery of astaxanthin from Haematococcus pluvialis biomass harvested by flocculation and water reusability. Science of The Total Environment 841, 156795. https://doi.org/10.1016/J.SCITOTENV.2022.156795 DOI: https://doi.org/10.1016/j.scitotenv.2022.156795
Mathimani, T., Mallick, N., 2018. A comprehensive review on harvesting of microalgae for biodiesel – Key challenges and future directions. Renewable and Sustainable Energy Reviews 91, 1103–1120. https://doi.org/10.1016/J.RSER.2018.04.083 DOI: https://doi.org/10.1016/j.rser.2018.04.083
Min, K.H., Kim, D.H., Ki, M.R., Pack, S.P., 2022. Recent progress in flocculation, dewatering, and drying technologies for microalgae utilization: Scalable and low-cost harvesting process development. Bioresour Technol 344, 126404. https://doi.org/10.1016/J.BIORTECH.2021.126404 DOI: https://doi.org/10.1016/j.biortech.2021.126404
Mubarak, M., Shaija, A., Suchithra, T. V., 2019. Flocculation: An effective way to harvest microalgae for biodiesel production. J Environ Chem Eng 7, 103221. https://doi.org/10.1016/J.JECE.2019.103221 DOI: https://doi.org/10.1016/j.jece.2019.103221
Muylaert, K., Bastiaens, L., Vandamme, D., Gouveia, L., 2017. Harvesting of microalgae: Overview of process options and their strengths and drawbacks. Microalgae-Based Biofuels and Bioproducts: From Feedstock Cultivation to End-Products 113–132. https://doi.org/10.1016/B978-0-08-101023-5.00005-4 DOI: https://doi.org/10.1016/B978-0-08-101023-5.00005-4
Ogbonna, C.N., Nwoba, E.G., 2021. Bio-based flocculants for sustainable harvesting of microalgae for biofuel production. A review. Renewable and Sustainable Energy Reviews 139, 110690. https://doi.org/10.1016/J.RSER.2020.110690 DOI: https://doi.org/10.1016/j.rser.2020.110690
Priya, T., Tarafdar, A., Gupta, B., Mishra, B.K., 2018. Effect of bioflocculants on the coagulation activity of alum for removal of trihalomethane precursors from low turbid water. Journal of Environmental Sciences 70, 1–10. https://doi.org/10.1016/J.JES.2017.09.019 DOI: https://doi.org/10.1016/j.jes.2017.09.019
Qi, S., Wang, Z., Hu, Y., Lei, J., Zhan, X., Stengel, D.B., 2023. Selective enrichment of auto-floating microalgae for wastewater bioremediation and biofuel/bioproduct production. Algal Res 69, 102911. https://doi.org/10.1016/J.ALGAL.2022.102911 DOI: https://doi.org/10.1016/j.algal.2022.102911
Ruggeri, M.V.R., Godoy, R.F.B., Arroyo, P.A., Trevisan, E., 2021. Evaluation of natural flocculant efficiency in the harvest of microalgae Monoraphidium contortum. SN Appl Sci 3, 1–9. https://doi.org/10.1007/S42452-021-04614-4/TABLES/3 DOI: https://doi.org/10.1007/s42452-021-04614-4
Salim, S., Kosterink, N.R., Tchetkoua Wacka, N.D., Vermuë, M.H., Wijffels, R.H., 2014. Mechanism behind autoflocculation of unicellular green microalgae Ettlia texensis. J Biotechnol 174, 34–38. https://doi.org/10.1016/J.JBIOTEC.2014.01.026 DOI: https://doi.org/10.1016/j.jbiotec.2014.01.026
Udayan, A., Sirohi, R., Sreekumar, N., Sang, B.I., Sim, S.J., 2022. Mass cultivation and harvesting of microalgal biomass: Current trends and future perspectives. Bioresour Technol 344, 126406. https://doi.org/10.1016/J.BIORTECH.2021.126406 DOI: https://doi.org/10.1016/j.biortech.2021.126406
Vandamme, D., Muylaert, K., Fraeye, I., Foubert, I., 2014. Floc characteristics of Chlorella vulgaris: Influence of flocculation mode and presence of organic matter. Bioresour Technol 151, 383–387. https://doi.org/10.1016/J.BIORTECH.2013.09.112 DOI: https://doi.org/10.1016/j.biortech.2013.09.112
Visigalli, S., Barberis, M.G., Turolla, A., Canziani, R., Berden Zrimec, M., Reinhardt, R., Ficara, E., 2021. Electrocoagulation–flotation (ECF) for microalgae harvesting – A review. Sep Purif Technol 271, 118684. https://doi.org/10.1016/J.SEPPUR.2021.118684 DOI: https://doi.org/10.1016/j.seppur.2021.118684
Vu, H.P., Nguyen, L.N., Emmerton, B., Wang, Q., Ralph, P.J., Nghiem, L.D., 2021. Factors governing microalgae harvesting efficiency by flocculation using cationic polymers. Bioresour Technol 340, 125669. https://doi.org/10.1016/J.BIORTECH.2021.125669 DOI: https://doi.org/10.1016/j.biortech.2021.125669
Wang, Q., Oshita, K., Takaoka, M., 2021. Flocculation properties of eight microalgae induced by aluminum chloride, chitosan, amphoteric polyacrylamide, and alkaline: Life-cycle assessment for screening species and harvesting methods. Algal Res 54, 102226. https://doi.org/10.1016/J.ALGAL.2021.102226 DOI: https://doi.org/10.1016/j.algal.2021.102226
Wang, S., Yerkebulan, M., Abomohra, A.E.F., El-Khodary, S., Wang, Q., 2019. Microalgae harvest influences the energy recovery: A case study on chemical flocculation of Scenedesmus obliquus for biodiesel and crude bio-oil production. Bioresour Technol 286, 121371. https://doi.org/10.1016/J.BIORTECH.2019.121371 DOI: https://doi.org/10.1016/j.biortech.2019.121371
Wang, S.K., Stiles, A.R., Guo, C., Liu, C.Z., 2015. Harvesting microalgae by magnetic separation: A review. Algal Res 9, 178–185. https://doi.org/10.1016/J.ALGAL.2015.03.005 DOI: https://doi.org/10.1016/j.algal.2015.03.005
Xu, K., Zou, X., Chang, W., Qu, Y., Li, Y., 2021. Microalgae harvesting technique using ballasted flotation: A review. Sep Purif Technol 276, 119439. https://doi.org/10.1016/J.SEPPUR.2021.119439 DOI: https://doi.org/10.1016/j.seppur.2021.119439
Yang, Z., Hou, J., Miao, L., 2021. Harvesting freshwater microalgae with natural polymer flocculants. Algal Res 57, 102358. https://doi.org/10.1016/J.ALGAL.2021.102358 DOI: https://doi.org/10.1016/j.algal.2021.102358
Yin, Z., Li, S., Hu, D., Li, Z., Chu, R., Liu, C., Li, X., Hu, J., Zhu, L., 2022. Performance evaluation of different chitosan-clay composite materials for efficient harvesting of Chlorella vulgaris and impacts on downstream bioproduct processing and water reusability. Chemical Engineering Journal 430, 132892. https://doi.org/10.1016/J.CEJ.2021.132892 DOI: https://doi.org/10.1016/j.cej.2021.132892
Zhao, Z., Li, Y., Muylaert, K., Vankelecom, I.F.J., 2020. Synergy between membrane filtration and flocculation for harvesting microalgae. Sep Purif Technol 240, 116603. https://doi.org/10.1016/J.SEPPUR.2020.116603 DOI: https://doi.org/10.1016/j.seppur.2020.116603
Zhu, L., Pan, G., Xu, H., Kong, L., Guo, W., Yu, J., Mortimer, R.J.G., Shi, W., 2021. Enhanced chitosan flocculation for microalgae harvesting using electrolysis. Algal Res 55, 102268. https://doi.org/10.1016/J.ALGAL.2021.102268 DOI: https://doi.org/10.1016/j.algal.2021.102268
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